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KMID : 0380619900220020134
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Korean Journal of Food Science and Technology
1990 Volume.22 No. 2 p.134 ~ p.139
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Purification and Characterization of β - galactosidase from Nuruk Yeast
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Abstract
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A strain of Nuruk yeast No. 15 (NY-15) which produced high activity of β-galactosidase was is olated from Nuruk, and the crude enzyme was prepared by whey permeate culture of the microorganism. The crude enzyme was purified 40-fold with a 7.7% yield by acetone and ammonium sulfate fractionational precipitation, and chromatography on DEAE-cellulose, DEAE-Sephadex A-50 and Agarose-PAPT. Purifiedβ-galactosidase from Nuruk yeast showed two types of subunit patterns; a slow moving band and a fast moving deeply stained band, both anode-migrating at pH 7.5. The molecular weight of the former was estimated to be about 130,000 and that of the latter was 96,000 by SDSpolyacrylamide gel electrophoresis. The optimum pH of the enzyme activity was 7.5 and maximum activity appeared at 40℃.
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